superoxide dismutase assay kit  (R&D Systems)

Journal: Acta Cirúrgica Brasileira

Article Title: Protective effects of fisetin on ovarian ischemia-reperfusion injury in rats via modulation of the TLR4-MyD88-TRAF6 signaling pathway

doi: 10.1590/acb405925

Figure Lengend Snippet: Effect of fisetin treatment on the antioxidant parameters in ovarian ischemia-reperfusion injury in rats. (a) malondialdehyde (MDA), (b) catalase (CAT), (c) glutathione peroxidase (GPx), (d) reduced glutathione (GSH), (f) superoxide dismutase (SOD). Results are shown as means ± standard deviation.

Article Snippet: The level of oxidative stress parameters such as catalase (CAT), GSH, glutathione peroxidase (GPx), superoxide dismutase (SOD) and MDA; cytokines tumor necrosis factor-α (TNF-α), interleukin-1β (IL-1β), interleukin-6 (IL-6), interleukin-10 (IL-10), interleukin-18 (IL-18); inflammatory parameters like cyclooxynase-2 (COX-2), iNOS, prostaglandin E2 (PGE2), nuclear kappa B factor (NF-κB), C reactive protein (CRP); apoptosis parameters viz., caspase-3, Bcl-2 and Bax were estimated using the ELISA kits following the manufacture instruction (R&D Systems, Minneapolis, MN, United States of America).

Techniques: Standard Deviation

Journal: Journal of Biological Methods

Article Title: Variations in the fetal bovine serum and glucose concentration in the culture medium impact the viability of glioblastoma cells as evidenced through the modulation of cell cycle and reactive oxygen species: An in vitro study

doi: 10.14440/jbm2025.0016

Figure Lengend Snippet: Variations in the antioxidant enzyme activity levels and expression, glutathione content, and mitochondrial membrane potential in C6 cells. Glioblastoma cells were starved and supplemented with different glucose concentrations (0 mg/dL, 100 mg/dL, and 400 mg/dL). The expression and activity of antioxidant enzymes and stability in mitochondrial membrane potential were assessed. (A) NQO1, (B) SOD, and (C) GPx activity in C6 cells in 0 mg/dL glucose (starved), 100 mg/dL, and 400 mg/dL glucose (starved and supplemented) conditions compared to 450 mg/dL glucose (unstarved) condition. Representative quantified images of blots depicting (D) NQO1 expression, (E) SOD expression, (F) GPx expression, and (G) GSH levels in 0 mg/dL glucose (starved), 100 mg/dL, and 400 mg/dL glucose (starved and supplemented) conditions compared to 450 mg/dL glucose (unstarved) condition. (H) Representative quantified images of JC-1 dye for mitochondrial membrane potential expression in 0 mg/dL glucose (starved), 100 mg/dL, and 400 mg/dL glucose (starved and supplemented) compared to 450 mg/dL glucose (unstarved). Scale bar: 72.7 μm, magnification: 20×. The data represent the mean of two independent experimental values with at least three replicate wells in each experiment. The statistical analysis was conducted using Tukey’s post hoc test. Statistical significance was determined at *** p <0.001. ns refers to not significant. Abbreviations: GPx: Glutathione peroxidase; GSH: Glutathione; NQO1: Nicotinamide adenine dinucleotide phosphate quinone oxidoreductase; SOD: Superoxide dismutase.

Article Snippet: Superoxide dismutase (SOD) activity in the serum was determined using a high-throughput SOD assay kit from R&D Systems (Cat No: 7501-500-K).

Techniques: Activity Assay, Expressing, Membrane

Journal: Journal of Biological Methods

Article Title: Variations in the fetal bovine serum and glucose concentration in the culture medium impact the viability of glioblastoma cells as evidenced through the modulation of cell cycle and reactive oxygen species: An in vitro study

doi: 10.14440/jbm2025.0016

Figure Lengend Snippet: Variations in the antioxidant enzyme activity levels and expression, glutathione content, and changes in mitochondrial membrane potential in U-87 MG cells. Glioblastoma cells were starved and supplemented with different glucose concentrations (0 mg/dL, 100 mg/dL, and 400 mg/dL). The expression and activity of antioxidant enzymes and stability in mitochondrial membrane potential were assessed. (A) NQO1, (B) SOD, and (C) GPx activity in U-87 MG cells under 0 mg/dL glucose (starved), 100 mg/dL, and 400 mg/dL glucose (starved and supplemented) conditions compared to 450 mg/dL glucose (unstarved) condition. Representative quantified images of blots depicting (D) NQO1 expression, (E) SOD expression, (F) GPx expression, and (G) GSH levels under 0 mg/dL glucose (starved), 100 mg/dL, and 400 mg/dL glucose (starved and supplemented) conditions compared to 450 mg/dL glucose (unstarved) condition. (H) Representative quantified images of JC-1 dye for mitochondrial membrane potential expression in 0 mg/dL glucose (starved), 100 mg/dL, and 400 mg/dL glucose (starved and supplemented) compared to 450 mg/dL glucose (unstarved). Scale bar: 72.2 μm, magnification: 20×. The data represent the mean of two independent experimental values with at least three replicate wells in each experiment. The statistical analysis was conducted using Tukey’s post hoc test. ns refers to not significant. Abbreviations: GPx: Glutathione peroxidase; GSH: Glutathione; NQO1: Nicotinamide adenine dinucleotide phosphate quinone oxidoreductase; SOD: Superoxide dismutase.

Article Snippet: Superoxide dismutase (SOD) activity in the serum was determined using a high-throughput SOD assay kit from R&D Systems (Cat No: 7501-500-K).

Techniques: Activity Assay, Expressing, Membrane

Journal: Journal of Biological Methods

Article Title: Variations in the fetal bovine serum and glucose concentration in the culture medium impact the viability of glioblastoma cells as evidenced through the modulation of cell cycle and reactive oxygen species: An in vitro study

doi: 10.14440/jbm2025.0016

Figure Lengend Snippet: Impact of fetal bovine serum (FBS) on the mitochondrial membrane potential and antioxidant enzymes activities and expressions in C6 cells. The cells were grown in complete media (4.5 g/L) and were then starved of FBS to synchronize the cultures. Subsequently, the cells were allowed to grow in a medium supplemented with different concentrations of FBS (0–10%) for 24 h. (A) NQO1 activity, (B) SOD activity, and (C) GPx activity in C6 cells cultured with 0% FBS (starved), 1–10% (starved and supplemented) compared to the cells cultured in a medium containing 450 mg/dL glucose in 10% FBS (unstarved). Representative quantified blots showing the expression of (D) NQO1, (E) SOD, and (F) GPx in C6 cells grown in 0% FBS (starved), 1–10% compared to the cells cultured in a medium supplemented with 450 mg/dL glucose and 10% FBS (unstarved). (G) GSH levels in C6 cells. (H) Representative photomicrographs of JC-1 dye-stained cells depicting the variations in mitochondrial membrane potential in C6 cells. Scale bar: 72.2 μm, magnification: 20×. The data represent the mean of two independent experimental values with at least three replicate wells in each experiment. The statistical analysis was conducted using Tukey’s post hoc test. Statistical significance was determined at * p <0.05, ** p <0.01, and *** p <0.001. ns refers to not significant. Abbreviations: GPx: Glutathione peroxidase; GSH: Glutathione; NQO1: Nicotinamide adenine dinucleotide phosphate quinone oxidoreductase; SOD: Superoxide dismutase.

Article Snippet: Superoxide dismutase (SOD) activity in the serum was determined using a high-throughput SOD assay kit from R&D Systems (Cat No: 7501-500-K).

Techniques: Membrane, Activity Assay, Cell Culture, Expressing, Staining

Journal: Journal of Biological Methods

Article Title: Variations in the fetal bovine serum and glucose concentration in the culture medium impact the viability of glioblastoma cells as evidenced through the modulation of cell cycle and reactive oxygen species: An in vitro study

doi: 10.14440/jbm2025.0016

Figure Lengend Snippet: Impact of fetal bovine serum (FBS) on the mitochondrial membrane potential and antioxidant enzymes activities and expressions in U-87 MG cells. The cultures were grown in complete medium supplemented with 4.5 g/L glucose and 10% FBS. Subsequently, the cells were starved and cultured in medium containing different concentrations of FBS (0–10%) for 24 h. (A) NQO1 activity, (B) SOD activity, and (C) GPx activity, in U-87 MG cells cultured with 0% FBS (starved), 1–10% (starved and supplemented) compared to the cells cultured in a medium containing 450 mg/dL glucose in 10% FBS (unstarved). Representative blots showing the expression of (D) NQO1, (E) SOD, and (F) GPx in U-87 MG cells cultured with 0% FBS (starved), 1–10% (starved and supplemented) compared to the cells cultured in a medium containing 450 mg/dL glucose in 10% FBS (unstarved). (G) Cellular GSH level. (H) Representative photomicrographs of cells stained with JC-1 dye for measuring the mitochondrial membrane potential. Scale bar: 72.2 μm, magnification: 20×. The data represent the mean of two independent experimental values with at least three replicate wells in each experiment. The statistical analysis was conducted using Tukey’s post hoc test. Statistical significance was determined at * p <0.05, ** p <0.01, and *** p <0.001. ns refers to not significant. Abbreviations: GPx: Glutathione peroxidase; GSH: Glutathione; NQO1: Nicotinamide adenine dinucleotide phosphate quinone oxidoreductase; SOD: Superoxide dismutase.

Article Snippet: Superoxide dismutase (SOD) activity in the serum was determined using a high-throughput SOD assay kit from R&D Systems (Cat No: 7501-500-K).

Techniques: Membrane, Cell Culture, Activity Assay, Expressing, Staining

Journal: Journal of Biological Methods

Article Title: Variations in the fetal bovine serum and glucose concentration in the culture medium impact the viability of glioblastoma cells as evidenced through the modulation of cell cycle and reactive oxygen species: An in vitro study

doi: 10.14440/jbm2025.0016

Figure Lengend Snippet: Effect of fetal bovine serum (FBS) on mitochondrial membrane potential and antioxidant enzyme levels in glioblastoma cell lines cultured in a medium containing no glucose or varied concentrations of glucose. Quantified images of (A) C6 cells and (B) U-87 MG cells treated with glucose and FBS and stained with JC-1. Red cells represent J-aggregates; green cells represent monomers. Merged images of red cells and green cells are presented in the third row. Scale bar: 72.2 μm, magnification: 20×. (C) NQO1 activity of C6 cells showed a non-significant decrease compared to cells treated with FBS (D) NQO1 activity of U-87 MG cells showed a significant decrease in activity at 24 h. (E) GSH activity of C6 cells remained high at 100 and 400 mg/dL in the presence of FBS. (F) GSH activity of U-87 MG cells showed a non-significant increase in FBS-treated glucose cells. (G) GPx activity of C6 cells remained unchanged. (H) GPx activity of U-87 MG cells at 24 h remained unchanged. (I) SOD activity in C6 cells was high at 400 mg/dL glucose. (J) SOD activity in U-87 MG cells showed no difference compared to the control. The values are presented as mean ± standard error of the means of two independent experimental values. The statistical analysis was conducted using Tukey’s post hoc test. Statistical significance was determined at * p <0.05, ** p <0.01, and *** p <0.001. ns refers to not significant. Abbreviations: GPx: Glutathione peroxidase; GSH: Glutathione; NQO1: Nicotinamide adenine dinucleotide phosphate quinone oxidoreductase; SOD: Superoxide dismutase.

Article Snippet: Superoxide dismutase (SOD) activity in the serum was determined using a high-throughput SOD assay kit from R&D Systems (Cat No: 7501-500-K).

Techniques: Membrane, Cell Culture, Staining, Activity Assay, Control